ABSTRACT

Medicinal plant is main source of cancer drug development. Melia azedarach is one of medicinal plant has been shown to have a strong cytotoxic activities in MCF-7 breast cancer cell line with IC50 20 μg/mL however, the cycle cell arrest effect of Melia azedarach was unknown. This study was aim to investigate the effect of Melia azedarach ethanolic extract on cycle cell arrest againts MCF-7 breast cancer cell line. In this quasy experimental study, MCF-7 were divided into 4 groups including control group and study groups (treated with the ethanolic extract of Melia azedarach at the dose 20 μg/mL, 10 μg/mL, 5 μg/mL). The cell cycle arrest effect was tested by propidium iodide. Accumulation of cell in each cycle cell phase were observed using flowcytometry. The difference among groups was analyzed using ANOVA with significant level 0.05. From qualitative image analysis data showing an increase in percentage of cell with a sub G0/G1 DNA content (p<0,05). In conclusion, Melia azedarach induces G0/G1 cycle cell arrest and apoptosis against MCF-7 breast cancer cell line

Keyword: Melia azedarach, apoptosis, cycle cell arrest, MCF-7 breast cancer cell line

        Kata kunci: