ABSTRACT

Medicinal plant is main source of cancer drug development. Melia azedarach is one of medicinal

plant has been shown to have a strong cytotoxic activities in MCF-7 breast cancer cell line with IC50

20 μg/mL however, the cycle cell arrest effect of Melia azedarach was unknown. This study was aim

to investigate the effect of Melia azedarach ethanolic extract on cycle cell arrest againts MCF-7 breast

cancer cell line. In this quasy experimental study, MCF-7 were divided into 4 groups including

control group and study groups (treated with the ethanolic extract of Melia azedarach at the dose 20

μg/mL, 10 μg/mL, 5 μg/mL). The cell cycle arrest effect was tested by propidium iodide.

Accumulation of cell in each cycle cell phase were observed using flowcytometry. The difference

among groups was analyzed using ANOVA with significant level 0.05. From qualitative image

analysis data showing an increase in percentage of cell with a sub G0/G1 DNA content (p<0,05). In

conclusion, Melia azedarach induces G0/G1 cycle cell arrest and apoptosis against MCF-7 breast

cancer cell line

Keyword: Melia azedarach, apoptosis, cycle cell arrest, MCF-7 breast cancer cell line

        Kata kunci: